Summary
Cyclic diguanylate (c‐di‐GMP) is a broadly conserved bacterial signaling molecule that modulates diverse cellular processes, such as biofilm formation, colony morphology, and swimming motility. The intracellular level of c‐di‐GMP is controlled by diguanylate cyclases (DGCs) with GGDEF domain and phosphodiesterases (PDEs) with either EAL or HD‐GYP domain. Pseudomonas putida KT2440 has a large group of genes on its genome encoding proteins with GGDEF/EAL/HD‐GYP domains. However, phenotypic‐genotypic correlation and c‐di‐GMP metabolism of these genes were largely unknown. Herein, by systematically constructing deletion mutants/overexpression strains of the 42 predicted c‐di‐GMP metabolism–related genes and analyzing the phenotypes, we preliminarily revealed the role of each gene in biofilm formation, colony morphology, and swimming motility. Subsequent results from protein sequence alignments and cellular c‐di‐GMP assessment indicated that 25 out of the 42 genes were likely to encode DGCs, nine genes were predicted to encode PDEs, four genes encoded bifunctional enzymes, and the other four genes encoded enzymatically inactive proteins. This study offers a basic understanding of the roles of these 42 genes and can serve as a toolkit for investigators to further elucidate the functions of these GGDEF and EAL/HD‐GYP domain‐containing proteins.