Shun Han, Shuang Tan, Achen Wang, Wenli Chen*, Qiaoyun Huang*.Deciphering belowground nitrifier assemblages with elevational soil sampling in a subtropical forest ecosystem (Mount Lu, China). FEMS Microbiology Ecology, 2019. ACCEPTED MANUSCRIPT.
Abstract
The elevational distribution patterns of microbial functional groups have long been attracting scientific interest. Ammonia-oxidizers (ammonia-oxidizing archaea [AOA] and bacteria [AOB]), complete ammonia oxidation (comammox) Nitrospira, and nitrite-oxidizers (e.g. Nitrobacter and Nitrospira) play crucial roles in the nitrogen cycle, yet their activities and abundances in response to elevational gradients in a subtropical forest ecosystem remain unclear. Here, we investigated the distribution of potential functions and abundances of these nitrifiers in forest soils along elevational gradients on Mount Lu, China. Our results showed that AOA and Nitrospira abundance was higher than that of their counterparts. Only AOA, Nitrobacter, and comammox Nitrospira abundances followed a hump-backed-model with the altitude. Soil potential ammonia-oxidation activity (PAO) and nitrite-oxidation activity (PNO) ranged from 0.003 to 0.084 and 0.34 to 0.53 μg NO2−-N g−1 dry soil h−1, respectively. The biotic (AOA, Nitrobacter, Nitrospira, and comammox Nitrospira abundances) and abiotic factors (soil variables) jointly affected PAO, whereas the abiotic factors were mainly responsible for PNO. Variance partitioning analysis showed that contemporary environmental disturbance is the most important driver for the biogeography of nitrifier assemblages. Overall, our findings indicate that forest soil nitrifier assemblages exhibit a biogeographic pattern majorly shaped by soil chemistry along an elevational gradient.
Keywords
Elevational gradients, forest soils, ammonia oxidizers, nitrite oxidizers, comammox Nitrospira, nitrifier assemblages